Ss Cyril and Methodius University, Republic of Macedonia
Title: The future in prevention of dental caries
Biography: Aleksandar Dimkov
Background: The antibacterial activity of conventional glass ionomer cement against three different microorganism strains alone and following incorporation of 1%, 2% and 3% benzalkonium chloride and cetylpyridinium chloride was evaluated.
Methods: The agar diffusion method was used to determine the inhibitory effect of the conventional glass ionomer cement ChemFlex on streptococcus mutans, lactobacillus casei and actinomyces viscosus. Bacterial strains were inoculated into BHIB and incubated in an anaerobic atmosphere (37°C). From the bacteria grown in the liquid medium, the density of the inoculum was set to be equivalent to McFarland 2 standard. In Shaedler agar, 350 uL of the bacterial suspension were equally spread. Specimens (4 mm - 96 mm) were prepared from the cement with and without addition of 1%, 2% and 3% benzalkonium chloride and cetylpyridinium chloride. The inhibition zones were determined after 48 h, after 7 days and after 21 days of incubation.
Results: The glass ionomer cements with no antimicrobial compounds incorporated either form very small inhibition zones or form no zones at all. The combination ChemFlex + Benzalkonium Chloride has the best effect on the three analyzed bacteria. The Benzalkonium Chloride antibacterial compound has a stronger antibacterial effect than Cetylpyridinium Chloride.
Conclusions: Glass ionomer cements can potentially be used as a medium for slow release of active antimicrobial components, and that have the potential to improve clinical outcomes of the cements.
- Gjorgievska E, Nicholson J W, Coleman N J, Booth S, Dimkov A and Hurt A (2017) Component release and mechanical properties of endodontic sealers following incorporation of antimicrobial agents. BioMed Research International 1:1-6.
- Dimkov A, Gjorgievska E, Stevanovic M and Nicholson W J (2016) Studies on the incorporation of benzalkonium chloride and cetyl pyridinium chloride antimicrobial agents into glass-ionomer dental cements. Research Journal of Pharmaceutical, Biological and Chemical Sciences 7(3):920-25.
- Dimkov A and Nicholson W J (2016) Releasing of fluoride ions from conventional glass-ionomer cements incorporated with antimicrobial agents. Asian Academic Research Journal of Multidisciplinary 3(3):267-87.
- Dimkov A, Gjorgievska E, Nicholson J W and Kaftandzieva A (2016) Antibacterial effects of conventional glass ionomer cement. Bratisl Med J. 117(1):31-35.